Butterfield Phosphate Diluent
Butterfield Phosphate Diluent is a sterile, isotonic phosphate-based solution used to dilute and preserve microbiological samples, particularly for pathogen testing in food, water, and environmental samples.
Chemical Name:
Phosphate Buffered Diluent (Sodium Phosphate, Monobasic/Disodium Phosphate with Potassium Chloride)
Synonyms:
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Butterfield’s Diluent
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Phosphate Diluent
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Butterfield’s Phosphate Buffer
CAS Number:
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Sodium Phosphate, Dibasic: 7558-79-4
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Sodium Phosphate, Monobasic: 13472-35-0
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Potassium Chloride: 7447-40-7
Appearance:
Clear, colorless aqueous solution

Key Properties:
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pH: ~7.2 ± 0.2
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Osmotically balanced to maintain microbial viability
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Sterile and free from contaminants
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Compatible with microbiological assays and sample dilution
Uses:
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Dilution of food, water, and environmental samples for microbiological testing
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Maintaining sample integrity prior to culture or detection
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Used in pathogen enumeration (e.g., Salmonella, Listeria)
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Laboratory microbiology research
Storage:
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Store at 2–8°C (refrigerated)
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Keep container tightly closed
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Avoid contamination after opening
Safety & Handling (SDS Summary):
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For laboratory use only
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Avoid contact with eyes and skin
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Use lab PPE: gloves, lab coat, goggles
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Wash hands thoroughly after handling
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Non-toxic, but avoid ingestion and contamination of food
| Weight | N/A |
|---|---|
| size | 1Lt, 2.5Lt, 5Lt |











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