
Urea Agar
Description:
Urea Agar is a differential medium used to detect the enzyme urease, produced by certain bacteria. It is especially used for the identification of Proteus, Helicobacter, Klebsiella, and some Yersinia species, which rapidly hydrolyze urea into ammonia and carbon dioxide. The resulting pH increase changes the color of the medium, providing a clear visual test result.
Type: Differential Microbiological Culture Medium
Appearance:
Dehydrated medium: light yellow to beige powder
Prepared medium: peach to light orange, semi-solid agar

Uses:
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Detection of urease activity in microorganisms
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Differentiation of Proteus spp. from other Enterobacteriaceae
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Diagnostic identification of Helicobacter pylori and Yersinia enterocolitica
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Routine biochemical testing in clinical microbiology
Key Features:
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Rapid visual detection of urease-positive bacteria
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Reliable color change indicator (phenol red)
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Easy to interpret and perform
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Standard component of IMViC and biochemical identification panels
Safety / SDS Summary:
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Hazards: Non-hazardous under normal laboratory use; avoid inhalation of dehydrated medium.
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First Aid: Rinse eyes or skin with water if contact occurs.
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Storage: Store dehydrated medium in a cool, dry place (15–25°C), away from moisture and light.
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Prepared medium: Store at 2–8°C, tightly sealed to prevent drying.
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Stability: Stable for long-term storage if protected from heat and moisture.
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